Fig. 6

Abundance of vsiRNAs specific to GRBV ORFs. A linearized depiction of the circular GRBV genome (5’ to 3’) showing ORF locations within the GRBV genome. Green, right-facing arrows indicate ORFs transcribed in the 5’-3’, viral sense. Red, left-facing arrows indicate ORFs transcribed in the 3’-5’, complementary sense. This depiction was created using SnapGene software [37] and the RefSeq GRBV annotation [22]. Abundance (RPM) of sRNAs which mapped to specific GRBV ORFs. Asterisks above individual bars indicate significant differences between pre- and post-véraison within a tissue type. In berries, sRNAs mapping to V1 were significantly less abundant and sRNAs mapping to C1-3 were significantly more abundant. In leaves, sRNAs mapping to C2 were significantly more abundant in post- relative to pre- véraison. Sampling categories are denoted according to the following abbreviations: pre-véraison [P], post-véraison [PO], leaves [L], berries [B], GRBV-positive [D]