Integrated analysis of tyrosine-induced MiRNA and mRNA expression profiles in melanocytes reveals the regulatory role of miR-1560-3p in melanin deposition in Xichuan black-bone chickens

Table 1 VIP gene 3’UTR wild and mutant type vector primer information

Primer Name	Primer Sequences(5’-3’)
WT F1	aattctaggcgatcgctcgagtagtatttcaaAGAGATGACTTTAGTCATCA
R1	attttattgcggccagcggccgcACTGGCATCCATATGTACAGCTTT
MT F2	aattctaggcgatcgctcgagtagtatttcaaCAGTCGAACTTTAGTCATCA

Note: Lowercase letters represent the homology arms of the primers, where the italicized bold part indicates the enzyme cut site and the capitalized bold part represents the mutation sequence

ISSN: 1471-2164