Fig. 5

Gonadal miRNAs markers identified in this study having had across evolution. miRNAs were filtered based on the heat-treated differential expressed (DEM, adjusted-p value < 0.05) data obtained in European sea bass, in the different comparisons based on sex and heat treatment. miRNAs were identified with log2 ratio>|1| of seed region expression in testis vs. ovary in at least five or more fish species, among which European sea bass. Furthermore, each miRNA was significantly differential expressed (DEM, adjusted-p value < 0.05) in our data. European sea bass DEM comparisons were: (1) Male high temperature (MHT) vs. Male control temperature (MCT); (2) Female high temperature (FHT) vs. Female control temperature (FCT); (3) MCT vs. FCT; (4) MHT vs. FHT. In purple boxes, the female-like miRNAs were represented and in green, the male-like miRNAs with log2 ratio>|1| of seed region expression in testis vs. ovary in at least five or more fish species