Fig. 3
From: The fate of artificial transgenes in Acanthamoeba castellanii
Southern blot detection of the neoR gene in transformed A. castellanii genomic DNA. The agarose gel on the left was used for the Southern blot on the right. Genomic DNA (gDNA) from A. castellanii transformed with circular pGAPDH-EGFP was run undigested (U) and two aliquots from the same sample were digested with each of two different restriction endonucleases, BglII (B) and HindIII (H). These endonucleases each have a 6 bp recognition site that occurs once within the plasmid. The purified pGAPDH-EGFP plasmid was run as a control, with the same three treatments. The ladder in the leftmost lane is Thermo Scientific GeneRuler 1 kb plus DNA ladder, with select band sizes marked alongside. Bright, diffuse nucleic acid signals can be observed at the bottom of each genomic DNA lane; these signals are thought to result from degraded and low molecular weight RNA that was not completely removed during DNA extraction. The full-length blot and gel are presented in Additional file 3