Table 1 Sequencing statistics for all nanopore long-read sequencing runs performed on A. castellanii transformants in this study
From: The fate of artificial transgenes in Acanthamoeba castellanii
Clone | Number of reads | Total bases | Estimated genome coverage | Mean read length (bp) | Mean read quality | Median read length (bp) | Median read quality | Read length N50 (bp) | Longest read (bp) |
|---|---|---|---|---|---|---|---|---|---|
Mixed transformants | 151506 | 1825008054 | 42X | 12045 | 11 | 9821 | 11 | 15287 | 83320 |
Clone 1 (shallow) | 124327 | 423236197 | 10X | 3404 | 8.8 | 1547 | 9.4 | 7662 | 60490 |
Clone 5 | 96988 | 289944147 | 7X | 2989 | 8.6 | 1360 | 9.2 | 6684 | 59098 |
Clone 8 | 83400 | 307873385 | 7X | 3691 | 8.6 | 1590 | 9.3 | 8837 | 68412 |
Clone 1 (deep) | 708542 | 7730398077 | 176X | 10910 | 10.9 | 5733 | 11.8 | 22989 | 393346 |
Clone LT6 | 440212 | 2569220207 | 59X | 5836 | 12 | 2739 | 12.1 | 13490 | 93794 |
Clone LT8 | 748516 | 4241519907 | 97X | 5666 | 11.9 | 4298 | 12.1 | 9560 | 154837 |
Clone LT9 | 388980 | 2353296042 | 54X | 6049 | 12.1 | 2536 | 12.2 | 15946 | 124150 |