Fig. 3

Bland-Altman analysis comparing RNA-Seq and NanoString platforms. (a) Bland-Altman plots for NHP-1 and NHP-2 at DPI 0, showing the mean differences and limits of agreement between the platforms. For NHP-1 at DPI 0, 97.95% of measurements fall within the 95% confidence limits (mean difference: 132.28), while for NHP-2 at DPI 0, 98.46% of measurements are within the limits (mean difference: 128.08). (b) The percentage of measurements within the 95% confidence limits ranges from 97.26–99.66% across all samples, with notable differences for NHP-4 at DPI 14 (mean difference: 527.83) and NHP-9 at DPI 21 (mean difference: 896.51), and smaller differences for NHP-2 at DPI 6 (mean difference: -31.86). “NHP-m-n” refers to NHP #m on day n post-infection