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Fig. 3 | BMC Genomics

Fig. 3

From: CircRNA profiling reveals circSMC1B that promotes bovine male germline stem cells proliferation and apoptosis via sponging let-7i

Fig. 3

Effect of circSMC1B on bovine mGSCs proliferation and apoptosis. a, b Visualization of pCD2.1-circSMC1B efficiency by RT-qPCR. c OD values were evaluated at 450 nm after CCK-8 addition. d PCNA, CDK2, CyclinD1, and p21 expression were detected by RT-qPCR. e-f The presence of EdU-positive cells was detected by the EdU kit in the proliferative phase. g-i Detection of cell cycle using flow cytometry and statistical analysis of cell numbers at various stages of the cell cycle, G1 phase, S phase. j After the addition of circSMC1B, detection of mRNA expression levels of cell apoptosis-related genes. k-l After circSMC1B activation, apoptotic mGSCs were detected by Annexin V-FITC/PI staining and flow cytometry. m Quantification of FITC-Annexin V/PI-positive cells presented in (k-l)

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BMC Genomics

ISSN: 1471-2164

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