Fig. 4
From: Optimizing Cost-Effective gene expression phenotyping approaches in cattle using 3′ mRNA sequencing
Volcano plots representing the differentially expressed genes of heat-stressed calves using (A) Takara libraries and (B) Lexogen libraries. The horizontal dashed line represents the adjusted p-value threshold of 0.05; all genes above the line are statistically significant. The vertical dashed lines represent \(\,\left|LFC\right|\ge\,1\); all genes beyond the vertical lines have moderate-to-high effect size as a response to heat stress. Differentially expressed genes (red dots) are both statically significant and have moderate-to-high effect sizes. (C) Number of DEGs detected by both library kits