Fig. 5

Variant calling results from Takara and Lexogen libraries. (A) Bar chart depicting differences in the number and location of variant sites. (B) Venn diagrams show the number and percentage of the variants called from the reads of each library preparation approach that are true population variants based on the ENSEMBL known variants GVF file. The two Venn diagrams on the left are when the variants are filtered more stringently (Alternate Allele Depth > = 10), and the two Venn diagrams on the right are when SNPs are filtered less stringently (Depth > 10 and Alternate Allele Depth > = 1). (C) A histogram representing the distribution of called intragenic SNPs across the gene body from 5′ → 3′ of the gene