Fig. 3

Within-sex comparison of female (A), male (B) and non-reproductive (C) specimens (columns; indicated by different colours) visualized by heatmaps representing the expression value [Centred log2(fpkm + 1)] of each differentially expressed transcript (lines) in each replicate. Yellow colours indicate higher expression values; purple colours indicate lower expression values. Trees on the top (samples) and left-hand side (transcripts) of each heatmap show hierarchical clustering based on similar expression patterns. Each column represents the expression pattern of a single replicate identified by an individual code (see Supplementary Files S6 to S11). Images on the right show the body regions included in each comparison: anterior end (top), midbody fragment (middle), and stolons (bottom). Images of the animals were modified from [37]. Arrowheads point towards individual oocytes (A) and sperm-filled segments (B). Scale bars: 2 mm (anterior end and midbody fragment), 500 µm (stolons)