Fig. 4

Across-sex comparison of the anterior end (A), the midbody region (B), and the stolons (C) (columns; indicated by different colours) visualized by heatmaps representing the expression value [Centred log2(fpkm + 1)] of each differentially expressed transcript (lines) in each replicate. Yellow colours indicate higher expression values; purple colours indicate lower expression values. Trees on the top (samples) and left-hand side (transcripts) of each heatmap show hierarchical clustering based on similar expression patterns. Each column represents the expression pattern of a single replicate identified by an individual code (see Supplementary Files S12, S13, S17, S18, S19 and S20). Images on the right show each body region: anterior end (A), midbody fragment (B), and female (left) and male (right) stolons (C). Images of the animals were modified from [37]. Scale bars: 2 mm (anterior end and midbody fragment), 500 µm (stolons)