Rapid, user-friendly, cost-effective DNA and library Preparation methods for whole-genome sequencing of bacteria with varying cell wall composition and GC content using minimal DNA on the illumina platform

Table 2 DNA Preparation methods

Library Preparation Kits	HS	GBD	EZ1	EZ1-AMP	Standard DNA Preparation Time*
Bacterial Liquid Culture	NA	NA	Overnight	Overnight	Overnight
Assay time (min/number of samples)	20 / 24	30–60 / 6	60–90 / 6	150–170 / 24	30–60 / 24

Caption: DNA preparations were initiated either directly from a colony culture (NA) or after overnight liquid culture at 37 °C. Assay time reflects the total duration required to obtain DNA ready for library preparation, starting from a bacterial colony. *Standard DNA preparation time based on literature [13]

ISSN: 1471-2164