Table 1 Venom proteins of C. congregata and other proteins and peptides overexpressed by venom glands
Category of venom protein | Name | Genbank accession number | Sub-category | Sequence length (number of amino acids) | Theoretical molecular weight (Da) | Theoretical pI | Level of overexpression in venom glands compared to ovaries (Fold change) | Presence of a predicted signal peptide | SignalP 6.0 score for “Other” | SignalP 6.0 score for SP | Cleavage site position and probability | Type of SP |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Venom proteins with predicted functions | 10-6 | CAD6231753.1 | Histidine phosphatase superfamily | 374 | 43748.95 | 5.61 | 5788.763269 | yes | 0.000267 | 0.999725 | CS pos: 21–22. Pr: 0.9745 | Sec/SPI |
Venom proteins with predicted functions | 80-6 | CAD6211813.1 | Serine carboxypeptidase (peptidase S10 family) | 415 | 47499.92 | 5.58 | 1872.589864 | yes | 0.000281 | 0.999701 | CS pos: 21–22. Pr: 0.9727 | Sec/SPI |
Venom proteins with predicted functions | 80-10 | CAD6208677.1 | Pheromone/general odorant binding proteins | 193 | 22119.92 | 4.52 | 85713.92416 | yes | 0.000197 | 0.999809 | CS pos: 20–21. Pr: 0.9793 | Sec/SPI |
Venom proteins with predicted functions | 90-3 | CAD6208091.1 | RAD52 family member | 211 | 23930.92 | 5.16 | 1319547.77 | yes | 0.000262 | 0.999718 | CS pos: 21–22. Pr: 0.9776 | Sec/SPI |
Venom proteins with predicted functions | 100-6 | CAD6225915.1 | Neprilysin (peptidase M13 family) | 701 | 82095.43 | 4.98 | 24765.48824 | yes | 0.000350 | 0.999615 | CS pos: 26–27. Pr: 0.9127 | Sec/SPI |
Venom proteins with predicted functions | vpcc1 | CAD6243299.1 | 5’-nucleotidase | 584 | 65496.22 | 5.55 | 62362.20861 | yes | 0.000253 | 0.999743 | CS pos: 21–22. Pr: 0.9723 | Sec/SPI |
Venom proteins with predicted functions | vpcc4 | CAD6208700.1 | Phosphodiesterases-like proteins of non eukaryotic origins | 316 | 36482.74 | 6.37 | 321311.6661 | yes | 0.000000 | 1.000000 | CS pos: 22–23. Pr: 0.9819 | Sec/SPII (Lipoprotein signal peptide) |
Venom proteins with predicted functions | vpcc5 | CAD6239998.1 | Pheromone/general odorant binding proteins | 147 | 16650.67 | 4.52 | 699840.3707 | yes | 0.000200 | 0.999777 | CS pos: 23–24. Pr: 0.5634 | Sec/SPI |
Venom proteins with predicted functions | vpcc7 | CAD6242938.1 | Proteins similar to bracoviral proteins | 117 | 13200.18 | 5.00 | 490389.0837 | yes | 0.000288 | 0.999671 | CS pos: 23–24. Pr: 0.5620 | Sec/SPI |
Venom proteins with predicted functions | vpcc8 | CAD6242940.1 | Proteins similar to bracoviral proteins | 121 | 13091.98 | 7.71 | 503132.3393 | yes | 0.000640 | 0.999343 | CS pos: 23–24. Pr: 0.7865 | Sec/SPI |
Venom proteins with predicted functions | vpcc12 | CAD6204022.1 | Phosphodiesterases-like proteins of non eukaryotic origins | 329 | 38618.35 | 8.70 | 111204.8963 | yes | 0.000297 | 0.999687 | CS pos: 25–26. Pr: 0.9754 | Sec/SPI |
Venom proteins with predicted functions | vpcc13 | CAD6204024.1 | Phosphodiesterases-like proteins of non eukaryotic origins | 325 | 37596.77 | 8.54 | 83137.64966 | yes | 0.000410 | 0.999580 | CS pos: 25–26. Pr: 0.9739 | Sec/SPI |
Venom proteins with predicted functions | vpcc17 | CAD6208082.1 | β-hexosaminidase | 594 | 67858.23 | 8.10 | 21165.55925 | yes | 0.000260 | 0.999736 | CS pos: 16–17. Pr: 0.9788 | Sec/SPI |
Venom proteins with predicted functions | vpcc19 | CAD6231748.1 | Histidine phosphatase superfamily | 378 | 44381.65 | 5.97 | 186909.9505 | yes | 0.000340 | 0.999641 | CS pos: 21–22. Pr: 0.9738 | Sec/SPI |
Venom proteins with predicted functions | vpcc24 | CAD6204019.1 | Phosphodiesterases-like proteins of non eukaryotic origins | 327 | 37001.13 | 7.62 | 20833.39251 | yes | 0.000394 | 0.999600 | CS pos: 25–26. Pr: 0.9686 | Sec/SPI |
Venom proteins with predicted functions | vpcc29 | CAG5101080.1 | Trypsin-like serine protease (peptidase S1 family) | 248 | 28531.61 | 5.20 | 38859971.48 | yes | 0.000249 | 0.999716 | CS pos: 18–19. Pr: 0.9781 | Sec/SPI |
Venom proteins with predicted functions | vpcc36 | CAD6216008.1 | Apolipoprotein D/lipocalin | 193 | 21489.05 | 4.55 | 17.98939646 | yes | 0.000201 | 0.999783 | CS pos: 19–20. Pr: 0.9799 | Sec/SPI |
Venom proteins of unknown functions | 80-4 | CAD6221875.1 | Protein with no predicted functional domain | 99 | 11183.81 | 6.86 | 4142901.67 | yes | 0.000184 | 0.999804 | CS pos: 18–19. Pr: 0.9812 | Sec/SPI |
Venom proteins of unknown functions | 90-9 | CAD6222053.1 | Protein with a predicted collagenase-like metallopeptidase catalytic domain | 299 | 34573.66 | 8.10 | 308360.539 | yes | 0.002927 | 0.997030 | CS pos: 20–21. Pr: 0.9105 | Sec/SPI |
Venom proteins of unknown functions | vpcc16 | CAD6242748.1 | Protein with no predicted functional domain | 275 | 31717.19 | 4.71 | 165169.1627 | yes | 0.350858 | 0.649131 | CS pos: 22–23. Pr: 0.4392 | Sec/SPI |
Venom proteins of unknown functions | vpcc20 | CAD6245297.1 | Proteins with tandem repeat DM9 domains | 410 | 45920.01 | 6.38 | 3022.723265 | yes | 0.000244 | 0.999753 | CS pos: 19–20. Pr: 0.9483 | Sec/SPI |
Venom proteins of unknown functions | vpcc21 | CAD6245301.1 | Proteins with tandem repeat DM9 domains | 412 | 46122.41 | 7.32 | 3593.906156 | yes | 0.000245 | 0.999739 | CS pos: 19–20. Pr: 0.9489 | Sec/SPI |
Venom proteins of unknown functions | vpcc30 | CAD6240076.1 | Proteins of the DUF4803 family | 358 | 41279.76 | 8.28 | 266505.9691 | yes | 0.211360 | 0.788612 | CS pos: 27–28. Pr: 0.7100 | Sec/SPI |
Venom proteins of unknown functions | vpcc37 | CAD6224830.1 | Cystein-rich protein | 363 | 41392.66 | 6.62 | 268621.1561 | yes | 0.000183 | 0.999799 | CS pos: 21–22. Pr: 0.9842 | Sec/SPI |
Venom proteins devoid of SP | vpcc31 | CAD6240116.1 | Proteins of the DUF4803 family | 389 | 44230.11 | 8.16 | 3917693.501 | no | 1.000000 | 0.000001 | NA | none |
Venom proteins devoid of SP | vpcc33 | CAD6240120.1 | Proteins of the DUF4803 family | 343 | 39144.95 | 5.57 | 2381275.61 | no | 0.999886 | 0.000133 | NA | none |
Venom proteins devoid of SP | vpcc34 | CAD6211217.1 | Protein with no predicted functional domain | 141 | 16204.26 | 4.63 | 1053121.155 | no | 1.000000 | 0.000000 | NA | none |
Venom proteins devoid of SP | vpcc35 | CAD6204869.1 | Protein with serpin domains | 400 | 45358.57 | 8.96 | 274982.7805 | no | 0.970902 | 0.029124 | NA | none |
Venom proteins devoid of SP | vpcc38 | CAD6218065.1 | Proteins with metalloprotease domains | 474 | 54694.26 | 8.40 | 139367.6789 | no | 0.955623 | 0.044395 | NA | none |
Venom proteins devoid of SP | vpcc39 | CAD6233576.1 | Proteins with metalloprotease domains | 546 | 64026.85 | 6.63 | 132722.9813 | no | 0.999977 | 0.000066 | NA | none |