Fig. 4

Structural analysis of the L643R mutation on the YrfF protein in E. coli K-12. A YrfF-WT (cyan, left) and YrfF-L643R (green, right) in the presence of the membrane. The mutated residue (Leu643 to Arg643) is highlighted in orange spheres in both structures. Red arrows represent the average angle of the periplasmic domain according to the normal of the bilayer (z-axis, black arrow). B Residues in YrfF forming hydrogen bonds with L643 (left) or R643 (right) are shown in orange. Residues in red (G389, V390, V392, D394, K630, I631, and F632) are predicted to lose membrane contact in YrfF-L643R but not in YrfF-WT