Fig. 1

SDS-PAGE analysis of waxy proteins in SM126 and its derivatives. (a) Identification of Wx-D1 protein subunit deletion mutant. White arrows indicate the present and absence of waxy proteins in the M4-6165 mutant. (M: Protein Marker; WT: Wild-type (SM126); Lanes 1–3: M4-6475 line; Lanes 4–6: M4-6165 line; Lanes 7–9: M4-6436 line; Lanes 10–12: M2-6166 line). (b) Hybridization of the F1 generation from the M4-6165 × M2-31 cross. (M: Protein Marker; WT: Wild-type (SM126); CS: Chinese Spring; Lanes 1–10: F1 hybrids of M4-6165 × M2-31). (c) Detection of different Wx protein subunits in SM126. (CS: Chinese Spring; Lanes 1–3: Lack of Wx-D1 protein (Wx-abD); Lanes 4–6: Lack of Wx-A1 protein (Wx-Abd); Lanes 7–9: Lack of Wx-B1 protein (Wx-AbD); Lanes 10–12: Lack of all Wx protein subunits (Wx-abd)