Fig. 2

Analysis of Wx-D1 gene sequences in wheat parental SM126 and mutant M4-6165 lines. The black boxes represent exon regions, and the lines represent introns. The regions targeted by the PCR primers are indicated by black arrows. The first amplified region spans from exon 1 to intron 5, and the second region spans from exon 5 to exon 11. The SNP in exon 4 and the associated amino acid variation are highlighted in red