Fig. 3

Identification of a KASP (Kompetitive Allele-Specific PCR) marker for four different Wx protein subunits in SM126.Genotyping of the M6 population, M4-6165, and M2-31 lines was performed using fluorescence signals for discrimination. The two clusters represent the genotypes of homozygous M4-6165 mutants and homozygous SM126 wild-type. Mutants M2-31 and M4-6165 (HEX-tagged, Wx-A1/Wx-D1 silenced) formed the heterozygous group