Table 1 Primer sequences for the study
Primers | Sequence (5′–3′) | Description |
|---|---|---|
TaWx-D1F | CACTACACTGCTCTCCTGCG | Wx-Abd amplification |
TaWx-D1R | AGCTTGAACTAGTAGGCGTGC | |
TaWx-D2F | CCCTACCCGCGACTTTAACG | |
TaWx-D2R | CTTCACGGTGGGTGGCGA | |
SM126-Wx-A-F | GAAGGTGACCAAGTTCATGCTGGAATGCGCTACGGAACGG | Allele-specific primers for TaWx-abD |
SM126-Wx-A-H | GAAGGTCGGAGTCAACGGATTGGAATGCGCTACGGAACGA | |
SM126-Wx-A-G | AAGAAGGACTGAAGGAGGATGC | |
SM126-Wx-D-F | GAAGGTGACCAAGTTCATGCTCGGCAGGCTGGAGGAGC | Allele-specific primers for TaWx-Abd |
SM126-Wx-D-H | GAAGGTCGGAGTCAACGGATTCGGCAGGCTGGAGGAGT | |
SM126-Wx-D-G | GAAGGTCGGAGTCAACGGATTCGGCAGGCTGGAGGAGT | |
TaWx-A1-qRT-F | GAAACCGCACCGATTCGA | Detection expression of Wx-abD |
TaWx-A1-qRT-R | CCAGGCGTCCTTGTACTGGTC | |
TaWx-D1-qRT-F | CTGGAGAAGGTCCGGGG | Detection expression of Wx-Abd |
TaWx-D1-qRT-R | TCGGCACTTCCAGCGCC | |
TaWx-1-qRT-F | GTACAAGGACGCCTGGGACA | Detection expression of Wx-abd |
TaWx-1-qRT-R | CGCGCTTGTAGCAGTGGAAG | |
TaWx-A1b-F | GAAGGTGACCAAGTTCATGCTCAGTGGCGCAAGCTTACGG | Allele-specific primers for Wx-abD |
TaWx-A1b-H | GAAGGTCGGAGTCAACGGATTGCAGGAAGAAACATTTATAAGAAGGCGT | |
TaWx-A1b-G | GCGCCCTGGAGCAAGACT | |
TaWx-D1b-F | GAAGGTGACCAAGTTCATGCTAGAAGCACGTCCTCCCAGTTCTT | Allele-specific primers for Wx-Abd |
TaWx-D1b-H | GAAGGTCGGAGTCAACGGATTGGCGGTCATCGACTTACCTTCCA | |
TaWx-D1b-G | AGAGTTAAGACTACAATGGTGCTCTTGT |