Fig. 2

Validation of the gene expression by RT-qPCR. Three randomly selected genes, XM_044654894.1, XM_044611652.1, and XM_044631664.1, were selected for an RT-qPCR analysis. Each treatment had three biological and three technical replicates. The RNA-seq (right-axis) and RT-qPCR (left-axis) data representing the five genes were significantly correlated (p < 0.05)