Allelic variation in a cluster of epsilon glutathione S-transferase genes contributes to DDT and pyrethroid resistance in the major African malaria vector Anopheles funestus

Table 2 Genetic parameters of coding regions for GSTe3, GSTe4 and GSTe6 in the four countries

Genes	Locality	N	S	h (Hd)	Syn	NSyn	π (k)	D	F*
*GSTe3*	Benin	5	0	1 (0)	0	0	0(0)	-	-
	Cameroon	5	10	3 (0.80)	9	1	8 (5.60)	1.92	1.25
	Uganda	4	2	2 (0.50)	2	0	1.4 (1.00)	-0.70	-0.60
	Malawi	6	9	2 (0.53)	7	2	7.14 (4.80)	0.01	1.61*
	Total	20	16	6 (0.70)	13	3	8.48 (5.70)	0.98	1.21
*GSTe4*	Benin	5	0	1 (0)	0	0	0(0)	-	-
	Cameroon	5	0	1 (0)	0	0	0(0)	-	-
	Uganda	5	0	1 (0)	0	0	0(0)	-	-
	Malawi	5	1	2 (0.60)	0	1	0.89 (0.60)	1.22	1.15
	TOTAL	20	4	3 (0.40)	1	3	2.47 (1.37)	0.63	1.12
*GSTe6*	Benin	5	0	1 (0.00)	0	0	0(0)	-	-
	Cameroon	5	2	2 (0.60)	0	2	1.79(1.20)	1.45	1.45
	Uganda	5	1	2 (0.50)	0	1	0.75(0.50)	-0.61	-0.61
	Malawi	5	2	3 (0.70)	0	2	1.49 (1.00)	0.24	0.24
	TOTAL	20	6	6(0.83)	0	4	8.48 (2.50)	1.48	1.25

N: number of sequences; S: number of polymorphic sites; h: number of haplotypes; hd: haplotype diversity; Syn: synonymous mutations; Nsyn: non-synonymous mutations; π: nucleotide diversity multiplied by 103; K: Average number of nucleotide differences; D: Tajima’s statistics; F*: Fu and Li’s statistics; *:< p value 0.05

ISSN: 1471-2164