Fig. 1
From: Large tandem repeats of grass frog (Rana temporaria) in silico and in situ
R. temporaria metaphase plates and karyotypes after FISH with the indicated TR probes. The most frequently occurring k-mers corresponding to the most conserved parts of the monomer sequences for the six most abundant TRs were used as labelled oligonucleotide probes for in situ mapping (Table 1). The additional probe (494A PCR) was prepared from genomic DNA by PCR because of its relatively long monomer (494 bp). The probe names are given in the upper left corner for each image. Hybridisation signals (green) from all TR probes were observed in the periCEN regions of some chromosome pairs, and the chromosomes were counterstained with DAPI (blue). Bar 10 μm