Fig. 4

Expression of recombinant CmegOBP99b and development of polyclonal antibodies against CmegOBP99b. a Expression profiles of OBPs family. The color scale on the right shows the FPKM values for each developmental stage from red (high) to white (low), standardized by min–max normalization. B1, B2, B3, B4 and B5 represent the wandering stag, the prepupal stage, the middle pupal stage, the late pupal stage and the newly emerged adults, respectively. b Developmental expression patterns of CmegOBP99b in different developmental stage of C. megacephala. c Tissue-specific expression patterns of CmegOBP99b in adult females. d The protein was mainly expressed in the inclusion body since the expression plasmid contained CmegOBP99b natural signal peptide. MW: protein marker; 1: inclusion body protein; 2: Quality control diagram of amplified and purified protein (SDS-PAGE). e Western blot assay showed the results of CmegOBP99b rabbit polyclonal antibodies, 1: CmegOBP99b rabbit polyclonal antigen protein (16.81 KDa). f WB analysis of CmegOBP99b polyclonal antibodies in different tissues. 1–3: abdomen, 4–6: thorax, 7–9: head plus antenna, 10–12: head