Fig. 10

The overlapping methylation sites in three bacterial genomes (A) Prevotella ruminicola 23, (B) Prevotella bryantii strain TS1-5, and (C) Fibrobacter succinogenes subsp. succinogenes S85, and (D) detected methylated numbers between two sequencing kits. The Australian dataset for sequencing kit analysis only included the data from the PowerFecal kit. The Australian dataset used the ligation kit SQK-LSK109 and the rapid sequencing kit SQK-RBK110.96; The Spanish dataset used the ligation sequencing kit SQK-LSK109 and the rapid sequencing kit SQK-RBK004. Data were basecalled under SUP mode. Dotted lines in boxplots link the same DNA sample. A t-test was used to compare the mean between two library preparation kits (An unpaired t-test for the Australian dataset and a paired t-test for the Spanish dataset)