Fig. 6

Kingdom relative abundances of (A) sequencing and (B) extraction kits. The Australian dataset for sequencing kit analysis only included the data from the PowerFecal kit. The Australian dataset used the ligation kit SQK-LSK109 and the rapid sequencing kit SQK-RBK110.96; The Spanish dataset used the ligation sequencing kit SQK-LSK109 and the rapid sequencing kit SQK-RBK004. The DNA extraction kit dataset only used samples sequenced by the ligation kit SQK-LSK109. Data were basecalled under SUP mode. A t-test was used to compare the means of different extraction and sequencing protocols (An unpaired t-test for the Australian dataset and a paired t-test for the Spanish dataset). P-values < 0.05: *; P-values < 0.01: **; P-values < 0.001: ***