Table 1 Bovine and microbiome data used in this study
Location | Sample type | Extraction method | Library protocol | Biological replicates | Technical replicates |
|---|---|---|---|---|---|
Australia | Rumen fluid | PowerFecal Pro | SQK-LSK109 (ligase-based) | 1 | 3 |
Rumen fluid | PowerFecal Pro | SQK-RBK110.96 (transposase-based) | 1 | 3 | |
Rumen fluid | DNeasy Plant Mini | SQK-LSK109 (ligase-based) | 1 | 3 | |
Rumen fluid | Puregene | SQK-LSK109 with EXP-NBD104 (ligase-based) | 1 | 3 | |
Spain | Rumen fluid | PowerSoil | SQK-LSK109 (ligase-based) | 4 | 1 |
Rumen fluid | PowerSoil | SQK-RBK004 (transposase-based) | 4 | 1 | |
Australia | Cattle ear punch | Puregene | SQK-NBD114.24 (ligase-based) | 15 | 1 |
Cattle ear punch | Puregene | SQK-RBK110.96 (transposase-based) | 15 | 1 |