Fig. 4

A The transcript structure and domains of FADS2 gene. B The tertiary structure diagrams of FADS2-T0 and FADS2-T2. proteins and the structure of the exon deleted in FADS2-T2 is represented in magenta. C Transmembrane region prediction results. The second transmembrane region in FADS2-T2 exhibits a shortened length compared to FADS2-T0. D Distinct features of FADS2-T0 compared to FADS2-T2 in endoplasmic reticulum. Exon deletion in FADS2-T2 induces amino acid changes within and adjacent to transmembrane domains. E Lipogenic differentiation of C3H10 T1/2 cells and oil red O staining. F qPCR for adipogenesis-related genes