Fig. 8

Normal mode analysis of the TLR4 and vaccine complex using the iMODS server. (A) B-factor values are compared to regional mobility ratings ranging from 0 to 1. (B) Each residue in the complex’s deformability index, with higher values indicating greater deformability. (C) Covariance map showing uncorrelated (white), correlated (red), and anti-correlated (blue) motions. (D) Eigenvalue: the smaller it is, the simpler it is to distort the complex. (E) Variance is inversely proportional to the eigenvalue. Green represents cumulative variances, whereas red represents individual deviations. (F) Elastic network, with darker grey patches indicating increased stiffness