Fig.1

The melanin deposition patterns and histomorphology observation of breast muscle in Silkies across 13 time points during the embryonic period. A Changes in melanin content during breast muscle development. The red line is melanin content detected by HPLC, and the blue line is the statistical proportion of melanin granules by FM staining. Data with different letters on each bar mean statistically significant difference among time points (p < 0.05). B HE staining of transversal breast muscle sections. C FM staining of transversal breast muscle sections. The green arrow points to blood cells, and the blue arrow points to melanin