Fig. 3

Integration of miRNA, mRNA and protein networks following 12 weeks of altered plane of nutrition in young bull calves. Differential expression of miR-2419-3p and C3 within the arcuate nucleus transcriptomes, indicated a relationship between these two transcripts, with miR-2419-3p up-regulated and C3 down-regulated in the HI calves. Co-regulatory network analysis of proteins identified within the arcuate nucleus revealed C3 as a hub protein, proteins connected to C3 by a red line indicate those regulating C3, whilst green lines indicate proteins being regulated by C3