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Fig. 3 | BMC Genomics

Fig. 3

From: The whole genome analysis of the wild-type and attenuated orf virus reveals that ORF022 facilitates viral replication

Fig. 3

Subcellular localization and transcriptional dynamics of ORF022. (A): Subcellular localization of ORF022. The green fluorescence represents the expression of ORF022 and GFP fusion proteins, while the blue fluorescence indicates the nucleus. B-C: Relative expression of FX0910 (B) and aFX0910 (C) ORF022 mRNA. GLF was collected at 12, 24, 48, and 72 h after infection with the same viral copy number of FX0910 and aFX0910 viruses, and RNA was extracted to detect ORF022 mRNA. (D-E): Copy number of FX0910 ORF022 (D) and FX0910 (E). GLF was collected at 12, 24, and 48 h after infection with FX0910, and DNA was extracted to detect copy number of ORF022 and FX0910. (F-G): Copy number of aFX0910 ORF022 (F) and aFX0910 (G). GLF was collected at 12, 24, 48, and 72 h after infection with aFX0910, and DNA was extracted to detect copy number of ORF022 and aFX0910. (**, P < 0.01, ***, P < 0.001, ****, P < 0.0001)

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BMC Genomics

ISSN: 1471-2164

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