Fig. 4
Effect of overexpressed ORF022 protein on ORFV (FX0910). ORF022 overexpression experiment: GLF were either mock-infected or transfected with pEGFP-022-FX0910 for 24 h and then infected with FX0910 for 24 h. A-B: Real-time analysis of ORFV mRNA (A) and copy number (B) in ORF022 overexpression experiment. (C): TCID50 of the cell supernatant in the ORF022 overexpression experiment. (D-E): Immunofluorescence of ORFV after overexpressing ORF022 (D) and Integrated Optical Density analysis (E). The green fluorescence represents the expression of ORFV, while the blue fluorescence indicates the nucleus. (*, P < 0.05, **, P < 0.01, ***, P < 0.001)