Fig. 5
Effect of interference with ORF022 protein on ORFV (FX0910). ORF022 interference experiment: GLF were infected FX0910 for 2 h and then transfected with either negative siRNA or ORF022 siRNA for 48 h. (A-B): Real-time analysis of ORF022 mRNA (A) and copy number (B) in ORF022 interference experiment. (C-D): Real-time analysis of ORFV mRNA (C) and copy number (D) in ORF022 interference experiment. (E): TCID50 of the cell supernatant in the ORF022 interference experiment. (F-G): Immunofluorescence of ORFV after interference with ORF022 (F) and Integrated Optical Density analysis (G). The green fluorescence represents the expression of ORFV, while the blue fluorescence indicates the nucleus. (H-I): Transmission electron microscopy of ORFV, FX0910 infected cells for 2 h and the cells were harvested after 48 h of mock transfection (H) or transfection with siRNA (I). IV, immature virion, MV, mature virion, CR, crescent. (*, P < 0.05, **, P < 0.01, ***, P < 0.001)