Fig. 3
The spectrum of putative transcriptional regulators among the DEX-induced gene expression patterns. The heatmap presents the statistical significance of enrichment of binding sites for transcription factors (TF) in the regulatory regions of genes from the clusters identified in this work. The information about TFs binding data were obtained from the ChEA 2022 database. The columns represent GR-responsive genes grouped in clusters labeled A to P. UP and DOWN are combined groups of all DEX-increased and DEX-decreased transcripts, respectively. For each cluster, the number of genes and the observed direction of the transcriptional regulation (‘ + ’ increase, ‘-’ decrease) are indicated. Statistically significant results are represented by colored squares: padj. < 0.2 (gray); padj. < 0.1 (light red); padj. < 0.05 (medium red); padj. < 0.01 (dark red); padj. < 0.001 (deep red). TFs were annotated as specified in the legend. The dots represent tissue co-localization between DEX-induced gene expression patterns and corresponding TF expression in at least one tissue associated with the gene cluster (as detailed in Supplementary Table 3). Up to 10 top statistically significant results per cluster with a minimum of two genes per term were included. Light green dots represent TF expression level in tissues based on GTEx data (mean CPM > 50) [34]. Dark green dots represent TF expression measured in our dataset (mean log2 value for the control group > 8). Full results are listed in Supplementary Table 3