Fig. 4

Heatmap of gene overlaps between the GR-dependent transcriptional patterns and literature-based GR-dependent gene lists for various tissues and cells. Lists of genes for tissues and cell types were extracted from transcriptomic studies of GR-dependent gene expression (PMIDs are provided in the rightmost column). The chi-square test was used to assess the overlap between the clusters of GR-regulated genes (x-axis) and literature (y-axis). χ² values were transformed in the following way: log₂(χ² + 1) and are displayed in each rectangle of the heatmap (white blocks—no overlap; red blocks—higher overlap). Values in brackets within each square indicate the number of overlapping genes. Statistically significant results are indicated as colored frames: p_adj < 0.01 (green frames); p_adj < 0.0001 (purple frames). The gene clusters are labeled at the top of the columns. For each cluster, the number of genes and the observed direction of the transcriptional regulation (‘ + ’ increase, ‘ − ’ decrease) are indicated. Cluster UP and cluster DOWN represent combined groups of all DEX-induced and DEX-decreased transcripts, respectively. The row description on the right of the heatmap provides details on the gene lists obtained from the literature ('+'represents upregulation and'−'represents downregulation). The gene lists presented on the heatmap are organized according to hierarchical clustering, indicated by both row and column dendrograms. An extended table summarizing gene list overlaps is included in Supplementary Table 2