Fig. 3

The CAR-downTSS region was prone to being accessed at mono-nucleosome level, enriched with active histone modifications, and incorporated with histone variant H2a.z. (A) Density plot showing the fragment size distribution of ATAC-Seq reads falling within cluster 5 (CAR-downTSS) and cluster 3. (B) Density plot showing the fragment size distribution of ATAC-Seq reads falling within cluster 5 (CAR-downTSS) and cluster 1. (C-D) Signal intensity of H3K27ac (C) and H3K4me3 (D) within CAR-downTSS and its correlation to ATAC-Seq signal. (E-F) Density plot showing the fragment size distribution of H3K27ac (E) and H3K4me3 (F) Cut&Tag sequencing reads falling within CAR-downTSS and cluster 3. (G-I) Signal intensity of H3K27me3 (G), H3K9me3 (H) and H2a.z (I) within CAR-downTSS and its correlation to ATAC-Seq signal. (J) Density plot showing the fragment size distribution of H2a.z Cut&Tag sequencing reads falling within CAR-downTSS and cluster 3