Table 1 Primer information of RT-qPCR
From: Molecular regulation of whole genome DNA methylation in heat stress response of dairy cows
Gene Name | Primer Sequences | Product Length |
|---|---|---|
DNLZ | F:5’-TGCCAGAACCACCACATCAT −3’ | 205 bp |
R:5’-GGGTGGAATCCTGGTCCTCA −3’ | ||
MeCP2 | f:5’-AGGCCATTCCCAAGAAACGA-3’ | 110 bp |
R:5’-CTCCTGAACGGACCGGATAG −3’ | ||
DNMT1 | f:5’-AGTTCGTGGTGGAGCAAGTA −3’ | 237 bp |
R:5’-GCTCCGCAAAGAAAGTGTCA-3’ | ||
DNMT3 A | F:5’-CATCCGGGTGCTGTCTCTAT −3’ | 160 bp |
R:5’-GTCCCCGACGTACATGATCT-3’ | ||
DNMT3B | F:5’-CGGCCTTTCTTCTGGATGTTTG −3’ | 123 bp |
R:5’-GTGAGCAGCAGACACTTTGAT 3’ | ||
GAPDH | F:5’- GGCGCCAAGAGGGTCAT −3’ R:5’- AGGCATTGCTGACAATCTTGAG −3’ | 109 bp |