Fig. 4

Diagram of CpV-BQ1 genome primer binding sites. The complete 165,454Â bp CpV-BQ1 genome with the primer binding sites used for genome validation. Primer binding sites are represented by arrows specific to the direction of amplification. Primer pairs 1F&1R, 2F&2R, 3F&3R, 4F&4R, and 5F&5R used to amplify 9,000Â bp regions in the genome indicated by blue rectangles. Primer pairs 1E&2E, 1E&3E, 2E&2E, and 2E&3E were used to PCR amplify across the genome ends (Supplementary Table 1)