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Table 2 The primers used in RT-qPCR

From: Identification of sex-biased and gonadal developmental miRNAs during critical windows of early gonadal differentiation in the Chinese giant salamander (Andrias davidianus)

Name

RTprimer(specific) Sequence (5'– > 3')

reverse primer

forward primers

let-7b-3p

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGGAAG

GTCGTATCCAGTGCAGGGT

ACCACCGCTATACAACCTACT

miR-10b-5p

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCACAAA

GTCGTATCCAGTGCAGGGT

AACACGCTACCCTGTAGAACC

miR-34c-5p

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGCAATC

GTCGTATCCAGTGCAGGGT

AACCGGAGGCAGTGTAGTTAG

miR-3529-3p

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTGGAAG

GTCGTATCCAGTGCAGGGT

AAGCGCCTAACAACAAAATCACTA

miR-7-5p

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACAAC

GTCGTATCCAGTGCAGGGT

AACCGGTGGAAGACTAGTGATT

novel_157

CAGTGCAGGGTCCGAGGTCAGAGCCACCTGGGCAATTTTTTTTTTT

CAGTGCAGGGTCCGAGGT

AACAAGATCCCACCGCTGC

novel_261

CAGTGCAGGGTCCGAGGTCAGAGCCACCTGGGCAATTTTTTTTTTT

CAGTGCAGGGTCCGAGGT

AACAGACACCTGGTGCTGTAGG

novel_28

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAAATC

GTCGTATCCAGTGCAGGGT

AACCGGAGGCAGTGTAGTTAG

novel_60

CAGTGCAGGGTCCGAGGTCAGAGCCACCTGGGCAATTTTTTTTTTT

CAGTGCAGGGTCCGAGGT

AACACGCATCTCAGCAGTGC

U6

AACGCTTCACGAATTTGCGT

AACGCTTCACGAATTTGCGT

CTCGCTTCGGCAGCACA