Fig. 1

Protein sequence alignment, phylogenetic tree, and domain structure display of GH1 domains in all GH1 proteins of P. trichocarpa. A Alignment of amino acid sequence of GH1 domains. The sites with asterisks or arrows above are the putative conserved basic binding sites of H1 proteins. B Neighbor-joining phylogenetic tree and domain architecture of GH1 domain proteins in P. trichocarpa. Phylogenetic tree was built with protein sequences of GH1 domains of P. trichocarpa GH1 proteins using MEGA 5.05 (bootstrap values of 1,000 replicate). C 3D model and conserved binding residues (in red) in GH1 domain of Potri.002G043100 using I-TASSER. Potri.002G043100 model was built using 4qlc.1.K template and 3.50 Å X-ray method with 0.39 sequence similarity, 0.26 coverage, 60–130 range